Hfe 7500 sigma. For the latter, a FNP concentration of 0.

Hfe 7500 sigma To make microgels, two polymer. 9%, M W nanoparticles stabilizes the single water-in-HFE-7500 emulsion droplets, and decreases the tension of the bare water-oil interface from 53 mN/m to 35 mN/m. HFE-7500 was purchased from 3M Company. 5 g, MW: 900 g/mol, Sigma-Aldrich, UK) and dissolved in a mixture of 50 mL HFE-7500 with 50 mL of (with a density of 950 kg/m3 and a viscosity of 20 cSt) was acquired from Sigma-Aldrich. Products Building Blocks Explorer Genes Papers Technical Documents Site 3M Novec 7500 Engineered Fluid _____ Page: 3 of 11 flammable/explosive vapor air mixture. 75lL of OptiPrep (D1556-250ML; Sigma) to the solution, followed by careful pipetting to mix and en-sure that single bacteria remain resuspended during the entire encapsulation process. K. The reaction mixture was then left stirring overnight at 85 °C under an argon atmosphere. RAN 008 was To make the agarose shell, packed PAA particles, 3. For both cell types, a cell Phase-transfer catalysts (PTC) and other chemicals were purchased from Sigma Aldrich. 05 g Novec HFE-7500 (3M, USA) containing 1 wt% of surfactant in millipore water (Synergy, Merck Millipore, Germany) that optionally contains 150 mM or 1 M NaCl (Sigma-Aldrich, USA). 1 buffer. 5% 008-FluoroSurfactant by pipetting 0. Oil was removed from below the emulsion such that less than 100 µl remained. 2. For both cell types, a cell The organic product was stripped from the HFE-7500 phase with a polar solvent, and the HFE-7500 phase was reused "as is" with satisfactory results through eight runs. e. Alternatively, 1H,1H,2H,2H-perfluorooctanol (Sigma-Aldrich) can be used Sigma-Aldrich: CAS 78560-45-9: HFE-7500 3M Novec: Fluorochem: CAS 297730-93-9: Pico-Surf (5% in Novec 7500) Sphere Fluidics: Product code F006: CaCl 2: Fluorochem: CAS 10043-52-4: Rinse the channels with HFE-7500 3M Novec until all channels are filled. A syringe at −1000 μL/h was used to produce a negative pressure at the waste collection to further ensure that unsorted drops flowed to waste. 606*Log(T[°C]+273. ) in the continuous phase. 22 μm) HFE-7500 fluid. (Danbury, CT, USA). g. 5-1 ml of 5% 008-FluoroSurfactant in HFE-7500 to 4. Bulk and Prepack available at Sigmaaldrich. 52 mN/m, a viscosity of µ oil = 1. Lastly, the outer oil phase was washed off with 1H,1H,2H,2H-Perfluoro-1-octanol (Sigma, St. 1 M Calcium-EDTA. 2µm. , HFE 7500 or FC 40) with the help of two different fluorosurfactants: a triblock copolymer consisting of two perfluoropolyether (PFPE) chains linked to a polyethylene glycol (PEG) and a PFPE-carboxylic acid surfactant (i HFE-7500 (3M 98-0212-2928-5) FC-40 (Sigma F9755) Perfluoro phase: 2% (w/v) PFPE -PEG PFPE in HFE 7500 BD 10 mL syringe + 21G1 1/2 needle, syringe filter 0. fluorinated oil (HFE-7500, 3 M), and the aqueous mixture were injected via two inlets of the channel by a pressure pump (Fluigent). 3M Novec 7500 Engineered Fluid is designed for heat transfer applications for use HFE-7500 (Novec Engineered Fluid, 3 M) was used as the oil substrate for EPD-based droplet actuation. Novec 7500 fluid shares many of the inertness and dielectric properties of perfluorocarbons (PFCs) and perfluoropolyethers (PFPEs), and is a viable option for replacing them in a wide Krytox 157FS(H) (50 g, MW: ~5000 g/mol, Dupont, Wilmington, DE, USA) was dissolved in 50 mL anhydrous HFE-7500 (Sigma-Aldrich, St. ) Its' excellent inerting and dielectric properties make it a viable replacement for higher GWP fluids such as PFCs and PFPEs Find HFE-7500 and related products for scientific research at Merck 3M Novec 7500 Engineered Fluid is a non-flammable fluid with a very low global warming potential (GWP). LOUIS MO 63103 UNITED STATES Telephone : +1 314 771-5765 Fax : +1 800 325-5052 1. Fluorocarbon oil was used as the continuous phase (HFE-7500 with 0. Louis, Missouri, USA) to achieve a water–oil–water three layers structure. $172. Since fluorocarbon (FC) oils such as FC-40 and HFE-7500 are commonly used in single-cell experiments, one must render the PDMS channels hydrophobic. 00 Pico-Surf® is a patented range of biocompatible surfactants that are used in a variety of microdroplet and picodroplet applications. 4 wt % of a commercially available PEG-based fluorosurfactant in HFE-7500) was supplemented with a negatively charged perfluoropolyether (PFPE) carboxylic acid fluorosurfactant (10. Because the OB1 flow controller delivers nearly any flow and pressure profile to (with a density of 950 kg/m3 and a viscosity of 20 cSt) was acquired from Sigma-Aldrich. for 75 pL the flow rates for oil and tartrazine solution were 40 µL/min and 10 µL/min, respectively. 1% Picosurf 1) and this was injected into the microfluidic device at a flow rate of 1000 μL/hr. Fluorescence-activated droplet sorting. Sign In to View Organizational & Contract Pricing. (Sigma-Aldrich, USA 3M™ Novec™ 7000 Engineered Fluid 3 3M™ Novec™ 7000 Engineered Fluid Typical Physical Properties Kinematic Viscosity Kinematic Viscosity [cSt] = Z-EXP(-0. A light yellow product was obtained after HFE-7500 3M (TM) Novec (TM) Engineered fluid; CAS Number: 297730-93-9; Linear Formula: C9H5F15O; find Fluorochem (Preferred Partner)-FLUH99C7AC83 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich Novec HFE-7500(Sigma Aldrich) containing 0. Novec 7500 Engineered Fluid (HFE-7500 oil) 3M Cat# Novec 7500 Neat (un-dissolved) 008-FluoroSurfactant RAN Biotechnologies Cat# 008-FluoroSurfactant Methoxy PEG thiol (PEG-SH) Sigma-Aldrich Cat Droplets were then demulsified by adding 1H,1H,2H,2H-perfluoro-1-octanol (Sigma Aldrich) dissolved in HFE-7500 (Novec) at 20% (v/v). 16-羟基十六碳酸 98%; CAS Number: 506-13-8; EC Number: 208-028-7; Synonyms: 16-羟基棕榈酸,杜松酸; Linear Formula: HO(CH2)15CO2H at Sigma-Aldrich The continuous oil phase, i. 005 (5 x 106: CFU*/ml) or 0. HFE-7500: 3M Novec: N/A: PBS buffer: Sigma-Aldrich: Cat# E607008: MEM: Sigma-Aldrich: Cat# M4655: CaCl2: Sigma-Aldrich: Cat#931144: Glucose: Sigma-Aldrich: Cat#D9434: L-Glutamine: -1-Trichlorosilane in HFE-7500. 1H, 1H, 2H, 2H-perfluoro-1-octanol of 100 µL (Sigma-Aldrich) and 200 The use of 1–1. HFE-7500 3M (TM) Novec (TM) Engineered fluid; CAS Number: 297730-93-9; Linear Formula: C9H5F15O; find Fluorochem (Preferred Partner)-FLUH99C7AC83 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich 3M™ Novec™ Engineered Fluid 7500 is a non-flammable fluid with a very low global warming potential (GWP. $291. During combustion. 5 % (v/v) acetic acid (Sigma Aldrich U. 3M™ Novec™ 7500. for 15 s horizontally and then 2 min vertically with a custom vortexer (Fluent BioSciences, FB0002776). Other chemicals used in this study were purchased from Sigma-Aldrich (St. 0. Dynabeads M-270 Epoxy was purchased from Thermo Fisher Scientific (Waltham, MA, USA). 5% sterile phenol red solution (Sigma-Aldrich, cat. Sigma-Aldrich (1) Sustainability. Applied Filters: Keyword:'hfe Aldrich® Market Select, your comprehensive solution for the identification and procurement of readily available screening compounds and building blocks. Hazardous Decomposition or By-Products Substance Condition Carbon monoxide. Epoxidation of HFP was carried out in pressure reaction vessels (Andrews glass) equipped with a thermocouple and pressure gauge. 5% v/v Pico-Surf as surfactant, black). and fluorinated oils were purchased from Sigma-Aldrich (St. 295*Z+0. All solutions were degassed for 15 minutes in the vacuum chamber prior use. 7 HFE-7500 fluorinated oil with 2% (w/w) de-protonated Krytox surfactant (DuPont, catalog no. 77 cSt) was purchased from 3M. It can be kept for several months at room temperature. 4 HFE-7100 Methoxyperfluorobutane Methoxynonafluorobutane Nonafluoromethoxybutane Formula : C 5 H 3 F 9 O Molecular weight : Sodium fluorescein and sodium alginate were purchased from Sigma-Aldrich (MO, USA). ) was used to break the emulsion, post crosslinking. Hydrophobic modification of the channels surface was achieved by injecting a solution of 1% (v/v) trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma) in HFE-7500 oil into the channels. Sigma surfaces for the HFEs investigated in this work predicted by conductor-like screening model for real solvents (COSMO-RS) analysis. Mechanical stability of emulsion drops Another stream of HFE-7500 oil at 1000 μL/h was introduced at the sorting junction to drive the drops to waste collection when the DEP force was off. 1H, 1H, 2H, 2H-perfluoro-1-octanol of 100 µL (Sigma-Aldrich) and 200 To study cell adhesion on different oil phases, wells of a 96-well plate were filled with 100 μL of HFE 7500, 2 w/w% Krytox in HFE 7500, 2 w/w% RAN in HFE 7500 and 0. 98021229285) Caution Avoid direct contact as it may cause irritation of skin, eyes and airways. 427) was mixed with HFE-7500 at different volume percentages, as described in the Results and Discussion section. Fluorinated oil Novec HFE-7500 (3 M) with 1% (w/w) of 008-Fluorosurfactant (RAN Biotechnologies). Two hundred and eighty microliters of 0. 4. In the semiconductor industry, it is ideal for automated test equipment (ATE) and 3M™ Novec™ 7500 is a non-flammable fluid with a very low global warming potential (GWP. United States Globally. After printing, the collected emulsion is broken with an equal volume of 20% (v/v) perfluoro-1-octanol (Sigma-Aldrich) in HFE-7500. 370533) in HFE-7500; the emulsions are then mixed by pipetting and Pico-Surf® (2% (w/w) in Novec™ 7500) £ 210. 24. Protect the tube from light with aluminum foil. Novec 7500 fluid shares many of the inertness and dielectric properties of perfluorocarbons (PFCs) and perfluoropolyethers (PFPEs), and is a viable option for replacing them in a wide Resorufin sodium salt, fluorescein and rhodamine 6G (Sigma-Aldrich) solutions were prepared by dissolution in millipore water or NaCl solutions. Afterward, ~ 10 µL of fluorinated oil (HFE 7500 with 2 wt % RAN-008 surfactant, RAN Biotechnologies) is aspirated into the pipette tip and subsequently pipetted out of the tip into a 200 µL PCR . , Cambridge, UK) surfactant. 5% ultra-low gelling temperature agarose (Sigma-Aldrich) solution, and HFE-7500 fluorinated oil (3 M) with 2% w/w PEG-PFPE amphiphilic block copolymer surfactant (008-Fluoro-surfactant, Ran Technologies) were injected into the bubble trigger device respectively at 700, 1000, and 6000 µl h –1 Novec 7000 Engineered Fluid is a greener alternative, Cryofreezing solution. FluoSurf (2%, w / w) in HFE-7500 was purchased from Emulseo (Pessac, France). 01 (10 x 107 CFU/ml), , which means cell concentration in the first suspension was 5 x 106 CFU/ml In this method, an aqueous droplet containing large unilamellar vesicles (LUVs) is stabilized within a fluorinated oil (i. HFE-7500 3M (TM) Novec (TM) Engineered fluid; CAS Number: 297730-93-9; Linear Formula: C9H5F15O; find Fluorochem (Preferred Partner)-FLUH99C7AC83 MSDS, 3M™ Novec™ 7500 Engineered Fluid is useful as a heat transfer liquid for cooling reactors in the pharmaceutical and chemical processing industries. Calibrate all pumps using the Novec-7500 setting and tare flow rates and pressure. Agarose and PEG solutions are injected into a droplet generation device (Figure S-1) with the oil (HFE-7500 fluorinated oil supplemented with 5% (w/w methoxy-nonafluorobutane; HFE-7100. adding OptiPrep density gradient medium from Sigma-Aldrich can reduce this problem. The electrode and moat channels are filled with 2 M NaCl solution. The resulting light-yellow product was mixed with Jeffamine XTJ 501 (3. A layer of droplets should be visible after sorting. 484431. 5% ionic Krytox in HFE 7500 oil 66 was added to the cell–bead mixture and vortexed at 3,000 r. Color. Fluorinated fluid HFE-7500 (3M, USA) carrying 0. Seems like HFE 7500 and FC-40 fluorinated oils are suitable for the task. Place it in a cooling block. 1 Materials. ~900) were purchased from Sigma-Aldrich. Paul, USA) containing 5% (w/w) 008-FluoroSurfactant (RAN Biotechnologies, Beverly, USA) was used as the continuous phase. All other analytical grade chemicals and reagents were purchased from Sigma-Aldrich (St. A total 500 μl of HFE-7500 with a distinct 3M™ Novec™ 7500 is a non-flammable fluid with a very low global warming potential (GWP. 2% To make the agarose shell, packed PAA particles, 3. As shown in Fig. 6. 5 mM Krytox). 3M™ Novec™ 7100 Engineered Fluid 04/12/23 Page 2 of 10 1. In the third step, the microdroplets from step 2 were collected in HFE 7500 with 2 vol% acetic acid for crosslinking purposes and we let the product stand still overnight. Make the RNP mix in a total volume of 19 μL by combining 5000 ng of pooled sgRNAs, 4. Louis, MO, USA) and mixed with excess oxalyl chloride (12. 3M Novec 7500 Engineered Fluid is designed for heat transfer applications for use in cooling of ion implanters, CVD machines or other electronic applications. Krytox 157FS(H) (50 g, MW: ~5000 g/mol, Dupont, Wilmington, DE, USA) was dissolved in 50 mL anhydrous HFE-7500 (Sigma-Aldrich, St. The solution was heated to 60oC and filled into a 1 ml syringe. Applied Filters: Keyword:'3M™ Novec™ 7500' Showing 1-2 of 2 results for "3M™ Novec™ 7500" within Products. 2% Ionic PEG-Krytox (FSH 157, Miller-Stephenson) and the outer phase was composed of 1% HFE-7500 3M (TM) Novec (TM) Engineered fluid; CAS Number: 297730-93-9; Linear Formula: C9H5F15O; find Fluorochem (Preferred Partner)-FLUH99C7AC83 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich After the spheroids were formed in the aqueous droplets in the droplet reservoir area, we used a 10% (w/w) solution of 1H,1H,2H,2H-Perfluoro-1-octanol (Sigma-Aldrich) in HFE-7500 to coalesce the droplets into continuous aqueous phase, to be able to move the released spheroids oil-free into the cultivation part of the chip. 6. The produced f-Au@SiO 2 was weighed and redispersed in HFE-7500 (Novec TM, 3M TM) as a model among other commercially available fluorocarbon oils, such as FC-40 and FC-70 (Fluorinert TM, 3M TM 0. DUPONT Krytox FSL 157 was obtained through a local supplier (ElbChemical Trading). (3% FS-HFE hereafter) solution by dissolving 1 g of 008-fluorosurfactant in 33 mL of Novec HFE-7500. 100 L of 1H, 1H, 2H, 2H-perfluoro-1-octanol (Sigma-Aldrich) and 200 μL of 200 μg/mL yeast total RNA solution (Ambion) were then flushed and the droplets were broken by vortexing the mixture. 51142-49-5) with 5% (wt/wt) PEG-PFPE surfactant to To study cell adhesion on different oil phases, wells of a 96-well plate were filled with 100 μL of HFE 7500, 2 w/w% Krytox in HFE 7500, 2 w/w% RAN in HFE 7500 and 0. 5% ultra-low gelling temperature agarose (Sigma-Aldrich) solution, and HFE-7500 fluorinated oil (3 M) with 2% w/w PEG-PFPE amphiphilic block copolymer surfactant (008-Fluoro-surfactant, Ran Technologies) were injected into the bubble trigger device respectively at 700, 1000, and 6000 μl h –1 HFE-7500 fluorocarbon oil was purchased from 3M (Maplewood, MN, USA). Sigma surfaces for the HFEs investigated in this work predicted by conductor-like screening model for real solvents (COSMO-RS) Finally, high molecular weight HFEs, such as HFE-7500 and HFE-7300, exhibit lower saturation pressures and high normal boiling points, being a clear disadvantage when choosing an ORC working fluid, HFE-7100, oxalyl chloride, anhydrous dichloromethane, Jeffamine ED-900, phenol red, trichloro(1H,1H,2H,2H-perfluorooctyl)silane, sodium HFE 7500 with 2% surfactant is used as spacer oil and droplet generation oil. This oil was introduced into the microfluidic channels at a volume sufficient to completely cover and wet the entire network. HFE-7500 was obtained from 3M (MN, USA). Sigma 1000, Labhut, UK) at flow rates between 1000 µl/h and 4000 μl/h. Single-stranded DNA primers and primer dimers were digested using 1 unit μL-1 ExoI (New England Biolabs) and 1 U μL-1 HinFI (Thermo Fisher Scientific). c. S2) with the oil (HFE-7500 fluorinated oil supplemented with Aqueous droplets float on HFE 7500 oil; therefore, sorted droplets accumulate at the air-oil interface in the collection tube. p. 00. solution of perfluorooctanol (Sigma-Aldrich, catalog no. 5% Pico-Surf (PS1, Sphere Fluidics, Ltd. Wash the beads in the aqueous upper phase twice with 1% Span-80 in hexane and three times with TBSET buffer. d. The aqueous solution flowed through the central inlet, whereas the oil phase was divided into two streams before it interacted with the aqueous phase at the cross-junction and split Glycerol (Sigma-Aldrich, catalog number: G5516) Mineral oil (Sigma-Aldrich, catalog number: M5904) (HFE-7500) with 0. This protocol forces the β -CD to precipitate, forming nanoparticles which remain stably suspended in the HFE 7500. Growth protocol The compressed liquid viscosities of heptafluoropropyl methyl ether (HFE-7000), methoxy-nonafluorobutane (HFE-7100), ethoxy-nonafluorobutane (HFE-7200), and 2-trifluoromethyl-3-ethoxydodecafluorohexane (HFE-7500) were measured using a vibrating- wire viscometer over the temperature range of (253 to 373) K and at pressures from (0. Louis, Missouri, USA), and then enclosed by alginate hydrogel. The agarose solution is warmed with a space heater during emulsification to prevent solid-ification. 3M Legacy 11 Digit Stock Number: 98-0212-2929-3 3M Material Number: 7100003723. The oil and reaction solutions were respectively injected into the two inlets of chip using an air pressure pump (OB1 MK2, Elvesys, Paris, France) to produce water-in-oil droplets First, 300 μl of HFE-7500 (3M) with 5% wt/wt surfactant (RAN Biotechnologies, 008-FluoroSurfactant) was loaded into a 1-ml low-dead-volume syringe (Air-Tite Products A1), and the syringe was Sigma-Aldrich: CAS 78560-45-9: HFE-7500 3M Novec: Fluorochem: CAS 297730-93-9: Pico-Surf (5% in Novec 7500) Sphere Fluidics: Product code F006: CaCl 2: Fluorochem: CAS 10043-52-4: red] and an oil phase in I oil (HFE 7500 3M Novec with 0. 151-4. Peer Reviewed Papers. For Alcohol contained in the product was removed using a rotary evaporator and lyophilization before use. Request a Bulk Order. Ammonium persulfate (APS), OptiPrep, fluorescein O-methacrylate, and N, N, N′, N′-trimethylene diamine The evolution of wicking height for HFE-7500 shows a slight difference from those of acetone and ethanol, as shown in Fig. The oil is removed from the tube using a syringe and acrylic acid (Sigma Aldrich) to promote EDC functionalization. com. 1 to 30) MPa. Propylene glycol monomethyl ether acetate. SU-8 3000 photoresist was purchased from Micro Resist Technology, 1H,1H,2H,2H-perfluorooctyltriethoxysilane and fluorinated HFE-7500 from Fluorchem, Pico-Surf from Sphere Fluidics, 1H,1H,2H,2H-perfluoro-1-octanol (PFO) from Sigma-Aldrich, normal human dermal fibroblasts (NHDF) from Lonza, fetal bovine serum (FBS) from Greiner Bio-one, methoxy-nonafluorobutane; HFE-7100. 025 g mL −1 FNPs in HFE 7500. Sorted droplets were recovered from the collection tubing by flushing 200 L of HFE 7500 fluorinated oil (3 M). Emergency telephone number 1-800-364-3577 or (651) 737-6501 (24 hours) SECTION 2: Hazard identification DuPont Krytox 157-FSH (a perfluoropolyether, MW 7000–7500) and 3M Novec HFE-7100 and HFE-7500 were purchased from Miller-Stephenson Chemical Co. 2 µm Generation of and Culture in Droplets: Fill channel with perfluoro phase: add 3 mL LB + 150 µL log phase bacteria (syringe 1) + 10 µL 1010 pfu/mL T7 phage in 3 mL 1x PBST These, however, have densities considerably higher than water, so the water droplets float, which makes it difficult to manipulate them for instance for re-injection. Formula Weight. Fluoro-Stop (Sigma Aldrich U. 22 Under these conditions, the dsGUV Mix 20% PFO with HFE 7500 to break the droplets to form the beads (see Note 2). 157FSH) is loaded into a 3 mL syringe. mRNA:cDNA hybrids were purified using Agencout AMPure XP Epidermal growth factor (EGF) and hydrocortisone for cell culture were obtained from Bio-Techné and Sigma Aldrich, respectively. ) Its' excellent inerting and dielectric properties make it a viable replacement for higher GWP fluids such as PFCs and PFPEs 3M™ Novec™ 7000 Engineered Fluid 3 3M™ Novec™ 7000 Engineered Fluid Typical Physical Properties Kinematic Viscosity Kinematic Viscosity [cSt] = Z-EXP(-0. The reaction mixture was heated to 65 ˝ C and stirred for The reaction mixture was loaded immediately into a 1-ml syringe backfilled with HFE-7500 fluorinated oil (3M, (Sigma-Aldrich, cat. 4 µm pore size cell culture inserts (Sterlitech, Auburn, Washington, United States) for 3, 5, or 10 on 0. 5% Krytox-PEG-Krytox surfactant. 31 cP, a density of ρ oil = 1. 5% (w/w) 008-FluoroSurfactant (RAN The precursor solutions were injected into the microfluidic device at a flow rate of 300 μL/hr, for CMC-SH, and 200 μL/hr for the PEG crosslinker. Inc. Sigma) to the droplets The oil phase (1. Carbon dioxide. Simple bright-field microscope with C-mount camera port, e. Agarose and PEG solutions are injected into a droplet generation device (Supplementary Fig. 8. Sigma-Aldrich. HFE 7500 (with a density of 1614 kg/m3 and a viscosity of 0. 3M Novec 7500 Engineered Fluid (HFE-7500 oil, 3 M; Novec, cat. ) Its' excellent inerting and dielectric properties make it a viable replacement for higher GWP fluids such as PFCs and PFPEs in a wide variety 3M Novec 7500 Engineered Fluid (HFE-7500) (Gallade Chemical, cat. 2, and 35. Physical Form. Ammonium hydroxide solution (28%) was provided by Acros Organics. Flow rates: I oil = 500 μL h −1; I CP = 300 μL h −1; I T Novec 7500 Engineered Fluid (HFE-7500 oil) 3M: Cat# Novec 7500: Neat (un-dissolved) 008-FluoroSurfactant: RAN Biotechnologies: Cat# 008-FluoroSurfactant: Methoxy PEG thiol (PEG-SH) Sigma-Aldrich: Cat# 729108: 1H, 1H, 2H, 2H-Perfluoro-1-Octanol (PFO) Sigma-Aldrich: Cat# 370533: Dulbecco’s Modified Eagle’s Medium - high glucose: Sigma-Aldrich: For this, two syringes (1 mL, Terumo) were filled with dyes (blue and yellow) diluted in water, and one syringe was filled with the continuous phase fluid composed of a mixture of 2:1 ratio by volume of HFE (Hydrofluoroether 7500 Novec, Sigma Aldrich) oil and 1. 1,3-Bis(trifluoromethyl)-5-bromobenzene (Sigma; refractive index n D = 1. SHH0002-500ML. Quick View. 5 , droplets were monodisperse prior to and after 70 min incubation at 50 °C, successfully achieving thermostable in vitro synthesis of sGFP in water-in-oil droplets for the first time, to the A 2-mm diameter stir bar (V&P Scientific) is added into the well during printing. For the latter, a FNP concentration of 0. To form an emulsion of acrylamide/bisacrylamide mix in oil, we used HFE 7500 perfluorinated oil (3M) with PFPE-PEG surfactant (kindly provided by Prof. Tubing of fluid reservoirs should be primed before connection to device. 5. It is a clear, colorless, and odorless liquid with a low surface tension and high chemical stability. 51142-49-5) with 5% (wt/wt) PEG-PFPE surfactant to in 50 mL anhydrous HFE-7500 (Sigma-Aldrich, St. coli ECJW922 in TB (Terrific broth) medium with OD600 (optical density) of 0. The surfactant solution is prepared in the HFE-7500 oil, a. 3M Novec HFE-7500 Engineered Fluid 40 lb Barrel 3M #7100003723. Novec 7500) (see Reagent setup) The nanoparticle solution was sealed by HFE 7500 (Sigma, St. Place the droplet generation oil in the oil pump (see Note 17) and the vials with HFE-7500 in both aqueous phase pumps. Syringe pumps—three dosing modules and a base module (Nemesys, Cetoni) at 2% dissolved in HFE 7500 fluorinated oil filtered using syringe filter at 0. 5 , droplets were monodisperse prior to and after 70 min incubation at 50 °C, successfully achieving thermostable in vitro synthesis of sGFP in water-in-oil droplets for the first time, to the Sigma surfaces for the HFEs investigated in this work predicted by conductor-like screening model for real solvents (COSMO-RS) analysis. Dry the channels with a stream of argon. Fluorinated oil (HFE, Novec 7500 3 M) with a surface tension of σ oil = 15. After gelation, the cell-laden microgels were collected by breaking the oil emulsion using 20 % 1H,1H,2H,2H-perfluoro-1-octanol (PFO, 370533, Sigma-Aldrich) in HFE-7500. 0, 41. Data visualization and triggering of sorting events were performed as previously described by Gielen et al. Commercially available Krytox 157-FSH (99. Connect the oil (HFE 7500, 1% v/v Pico-Surf) and aqueous solution reservoirs to proper inlets of the microfluidic chip. After leaving the mixture to rest for 12 h, we remove the DMF fl oating on top of the HFE 7500 and fi lter the remaining HFE 7500 solution with a 250 nm-pore syringe fi lter. We measure the interfacial tension in 1 s intervals. 7487-3. The fluorinated oil HFE 7500 was purchased Fluorochem, Uranyl acetate from Electron Microscopy Sciences. The other two syringes, filled with an aqueous solution (height: 80 µm; width: 50 µm at the flow focusing junction) using HFE-7500 fluorinated oil and 2% 008-FluoroSurfactant (RAN biotechnologies). 06 g ethyl- 4(dimethylamino)benzoate (Merck), 0. 15% DMF solution to 85% HFE oil. The droplets containing cell colonies are reinjected into the droplet merger device. 3M’s Legacy 11- Digit Stock Number: 98-0212-2928-5 Manufacture Number: 7100025016. Select a Size. Contrarily, HFE-7500 has 15 F-atoms, but quite The reaction mixture was loaded immediately into a 1-ml syringe backfilled with HFE-7500 fluorinated oil (3M, (Sigma-Aldrich, cat. Available for Sale. 4vol%) consisted in dissolving a PEG-based fluorosurfactant in HFE 7500 oil. Agarose and PEG solutions are injected into a droplet generation device (Figure S-1) with the oil (HFE-7500 fluorinated oil supplemented with 5% (w/w Collected droplets were recovered by flushing 200 µL of surfactant-free HFE 7500 fluorinated oil (3 M) through the tubing. » Collection sample: 2% dSURF (Fluigent) with 0. It can be kept for several months at room 3 Introduction 3M™ Novec™ 7500 Engineered Fluid is a nonflammable fluid with very low global warming potential for use in heat transfer applications. 5-9 ml HFE-7500. 1 L. The system was primed by running both fluids at 2 bar Hydrofluoroether (HFE) 7500 oil (3 M, St. 3. 10. 5 μL of 10× r3. 3193*Z3) where: Z=10^(10^(10. For droplet generation process we used the RayDrop droplet generator. Louis, MO) unless otherwise stated. 05% surfactant (Pico-Surf, Sphere Fluidics) could also be added in HFE-7500 to adjust the These, however, have densities considerably higher than water, so the water droplets float, which makes it difficult to manipulate them for instance for re-injection. Phosphate buffer saline (PBS) Fluosurf in HFE- 7500 and the dispersed phase pump with 2 % (w/v) Alginate with 0. The shell liquid (polymer phase) comprised of 0. 5 g, Sigma-Aldrich). CellCarrier-96 Ultra Microplates, tissue culture treated were purchased from PerkinElmer. Encapsulated ACs and ACPCs were cultured in 6-well plates loaded with 0. 2 μL of 20 μM EnGen Spy Cas9 NLS, and 2. 00 – £ 930. FluoSurf 2wt% in HFE-7500 was obtained from Dolomite Microfluidics (Royston, UK). Ultra-low Gelling Temperature agarose, type IX-A (Sigma-Aldrich, catalog number: A2576) 1H,1H,2H,2H-Perfluoro-1-octanol (PFO), 97% (Sigma-Aldrich, catalog number: 370533) (HFE-7500) with 0. 63 g/ml and a boiling point of 128° C was used as the liquid phase for the core (oil phase) (Rausch et al. One of the biggest challenges of working simultaneously with millions 3M™ Novec™ 7100 Engineered Fluid 04/12/23 Page 2 of 10 1. The moat channel prevents stray fields from causing unintended droplet coalescence at other locations on the device . 5% or 3% dSURF (Fluigent, P/N DR-RE-SU-A1) Dispersed phase Reagents: 500 µl suspension of E. This process is an attractive prototype for the large-scale use, recovery, and reuse of fluorous organocatalysts. Please Note: 3M have announced their decision to discontinue their range of 3M Novec HFE 7500 Engineered Fluid 12 lb / 1 Gal Bottle 3M 7100025016. HFE-7500 is primarily used as a cleaning 3M™ Novec™ Engineered Fluid 7500 is a non-flammable fluid with a very low global warming potential (GWP. In both cases, the oil is covered with a layer of water. were purchased from Sigma-Aldrich (MO, USA). hfe 7500. Sigma-Aldrich: Cat# 729108: 1H, 1H, 2H, 2H-Perfluoro-1-Octanol (PFO) Sigma-Aldrich: Cat# 370533: Dulbecco’s Modified Eagle’s Medium - high glucose: Sigma-Aldrich: 2% HFE-7500 oil, (2) outlet for collection, (3) outlet for collection, (4) single clone suspension and (5) RT lysis mix. 05% surfactant (Pico-Surf, Sphere Fluidics) could also be added in HFE-7500 to adjust the ABSTRACT The liquid density, liquid kinematic viscosity, and surface tension of the segregated hydrofluoroethers (HFEs) HFE-7000 (1,1,1,2,2,3,3-heptafluoro-3-methoxy DuPont Krytox 157-FSH (a perfluoropolyether, MW 7000–7500) and 3M Novec HFE-7100 and HFE-7500 were purchased from Miller-Stephenson Chemical Co. 6119*Z2-0. Application. 2 Flow EZ were connected to alginate solution, Collected droplets were recovered by flushing 200 µL of surfactant-free HFE 7500 fluorinated oil (3 M) through the tubing. 500 ML. (F) Collection plate. . 025 g mL −1 was used to enable bright-field imaging through the oil layer. 15)))-0. HFE-7500 (Novec Engineered Fluid, 3 M) was used as the oil substrate for EPD-based droplet actuation. Novec 7500 fluid shares many of the inertness and dielectric properties of perfluorocarbons (PFCs) and perfluoropolyethers (PFPEs), and is a viable option for replacing them in a wide Time-lapse photographs of fluorinated oil (HFE-7500) (a) with 5 mM FSH 2-Jeffamine900 and (b) without surfactant. melting temperature agarose (Sigma-Aldrich) is used for agarose particle generation. 1 M calcium-EDTA (Sigma Aldrich) as the dispersed phase, collecting the product in a solution of the continuous To make the agarose shell, packed PAA particles, 3. , Leica DMI LED. Fill two glass vials with 10 mL sterile-filtered (0. 2015). 14 A detailed protocol is provided in the SI. Also is a non-flammable fluid Emulsions are broken using 2 mL of a 10% (v/v) solution of perfluoro-octanol (Sigma-Aldrich, catalog no. For all measurements, the oil phase was composed of HFE-7500 (Sigma) and 2. Flow rates were adjusted to the desired droplet volume e. Sylgard™ with HFE-7500 containing 2 wt% EA-surfactant, was connected to an oil inlet at the top of the generator. Epoxidation of HFP. Sigma-Aldrich ® Solutions of low melting Agarose (Sigma Aldrich A2576) was prepared in water. (Sigma-Aldrich) and Aqueous droplets float on HFE 7500 oil; therefore, sorted droplets accumulate at the air-oil interface in the collection tube. TA (99. Novec 7500 fluid shares many of the inertness HFE-7500 is a fluorinated solvent produced by 3M. for 15 s horizontally and then 2 min vertically with a Sigma-Aldrich (2) Sustainability. 7. These two solutions were then placed in separate 1-mL syringes (BD-309628; VWR) that were preloaded with 300lL of HFE-7500 oil (Novec 7500; 3M). However, the cost of the surfactants that I could find was very Alginate droplets were formed using 1 % v/v FluoSurf in HFE 7500 (Dolomite Microfluidics) as the continuous phase and 2 % w/v alginate in 0. To trigger the charge-mediated fusion of the SUVs and the formation of dsGUVs, the oil phase (containing 1. Technique. 3050 SPRUCE ST ST. Add to Cart. m. Garstecki). 7(c), the time duration for HFE-7500 capillary rise from the bottom to the top end of the DTWs are much longer than those of acetone and ethanol due to the lower wicking velocity. Fluorescent polystyrene beads with a diameter of 10 μm were (Shanghai, China). A light yellow product was obtained after solvent removal by rotary evaporation and high vacuum. Novec 7500) Caution Avoid direct contact; it may cause respiratory, skin, and eye irritation. We would like to show you a description here but the site won’t allow us. no. 5 % ≤) and 1 M of NaOH (sodium hydroxide) were purchased from sigma Aldrich. 5% ultra-low gelling temperature agarose (Sigma-Aldrich) solution, and HFE-7500 fluorinated oil (3 M) with 2% w/w PEG-PFPE amphiphilic block copolymer surfactant (008-Fluoro-surfactant, Ran Technologies) were injected into the bubble trigger device respectively at 700, 1000, and 6000 μl h –1 The reaction mixture and HFE 7500 fluorinated oil (3 M) with 2% (w/w) PEG-PFPE amphiphilic block copolymer surfactant (Ran Biotechnologies) are loaded into separate 1 ml syringes and injected at Sigma-Aldrich) and dissolved in a solvent mixture consisting of HFE 7500 (50 mL) and anhydrous dichloromethane (50 mL, Sigma-Aldrich). Emergency telephone number 1-800-364-3577 or (651) 737-6501 (24 hours) SECTION 2: Hazard identification Merck/Sigma: Cat# C9407: Dispase II: Thermo Fisher Scientific: Cat# 17105-041: DNAse (Deoxyribonuclease I from bovine pancreas) Merck/Sigma: Cat# DN25: Fetal bovine serum: Merck/Sigma: HFE-7500 3M (TM) Novec (TM) Engineered fluid: Fluorochem: Cat# 051243; CAS: 297730-93-9: Pico-Surf (5% (w/w) in Novec 7500) Sphere Fluidics: Cat# C022 solution (Sigma Aldrich) dissolved at 20% v/v in dSURF or HFE 7500 fluorinated oil Droplet generation process: The droplet generation set-up is illustrated in the previous picture. 4% (v/v) TEMED (Aladdin Bio-Chem Technology, Shanghai, China) and 1. 1 M calcium-EDTA (Sigma Aldrich) as the The use of 1–1. Louis, MO, USA), unless stated 1. HFE (hydrofluoroether, Novec 7500, purity grade: standard) was obtained from 3 M and Krytox 157 FSH was purchased from Chamours. 7 Oil was removed from the bottom of tubes and 20% 1 H, 1 H, 2 H, 2 H-perfluoro-1-octanol (PFO) (Sigma-Aldrich, 370533) in HFE-7500 oil was added at a ratio of 3:1 (v/v) PFO:emulsion. 370533) in HFE-7500; the emulsions are then mixed by pipetting and centrifuged at 300 g for 1 min. 5% RAN 008 fluorosurfactant in HFE 7500 provided the best droplet stability during both flow focusing and incubation. This product was then mixed with 2. Contrarily, HFE-7500 has 15 F-atoms, but quite Sesame oil (with a density of 919 kg/m 3 and a viscosity of 34 cSt) was procured from a local store, while silicone oil (with a density of 950 kg/m 3 and a viscosity of 20 cSt) was As a model oil, we employ a perfluorinated oil (HFE 7500) that is commonly used in microfluidic devices to produce emulsions (vinyl alcohol), and Pluronic surfactant were purchased from Alginate droplets were formed using 1 % v/v FluoSurf in HFE 7500 (Dolomite Microfluidics) as the continuous phase and 2 % w/v alginate in 0. Filter the beads through a 70 μm cell strainer and store them in TET buffer for up to 6 months at 4 °C (see Note 3). biocompatible oil phase, at 2% and 4% by weight. For 3 Introduction 3M™ Novec™ 7500 Engineered Fluid is a nonflammable fluid with very low global warming potential for use in heat transfer applications. 05% v/v acetic acid (Sigma Aldrich) » Emulsion breaker: Perfluoroctanol (PFO) solution (Sigma Aldrich) (20% v/v in HFE 7500 fluorinated oil) Products/Instrument: complete transfer using HFE 7500 as the carrier fluid and 008-fluorosurfactant as a surfactant. Feature. P0290-100ml) Novec HFE-7500 engineered fluid (3M, cat. 5% Sterile Phenol Red solution (Sigma-Aldrich). Hydrogen Fluoride During combustion. HFE-7500 3M (TM) Novec (TM) Engineered fluid; CAS Number: 297730-93-9; Linear Formula: C9H5F15O; find Fluorochem (Preferred Partner)-FLUH99C7AC83 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich added 4. The emulsion is amplified using 1 × KAPA HiFi HotStart ReadyMix (Roche) and 1 μM of forward primer and reverse primer (IDT). The PA intensity was enhanced since the absorption of laser light was 3 Introduction 3M™ Novec™ 7500 Engineered Fluid is a nonflammable fluid with very low global warming potential for use in heat transfer applications. 3. Louis, MO, USA) HFE-7500 3M (TM) Novec (TM) Engineered fluid; CAS Number: 297730-93-9; Linear Formula: C9H5F15O; find Fluorochem (Preferred Partner)-FLUH99C7AC83 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich DuPont Krytox 157-FSH (a perfluoropolyether, MW 7000–7500) and 3M Novec HFE-7100 and HFE-7500 were purchased from Miller-Stephenson Chemical Co. A second syringe was filled with fluorocarbon oil (HFE-7500) with 1. Louis, MO, USA). In Stock Details. These two syringes were attached to one end of PEEK tubing, and the other end of the Approximately 200 μL of collected droplets were then subjected to 2 minutes of UV irradiation and subsequently treated with 20%(v/v) PFO in HFE-7500 (Sigma-Aldrich, 370533-25G) to form HetHydrogels. ) Its' excellent inerting and dielectric properties make it a viable replacement for higher GWP fluids such as PFCs and PFPEs in a wide variety 3M™ Novec™ 7500 Engineered Fluid is a nonflammable fluid with very low global warming potential for use in heat transfer applications. However, the water-in-HFE We usually buy materials from Sigma-Aldrich (Merk). Company : Sigma-Aldrich Inc. The agarose solution is warmed with a space heater during emulsification to prevent solidification. while the yields from HFE-7100, HFE-7200, and HFE-7500 solvent systems were 37. fqelww mqhkyk ully afmnf gbmrfwj uhqsjop lkm ful yljr jvthee